Gene name Ensembl gene id Ensembl transcript id Guide coordinates Guide midpoint Guide sequence ddCt ddCt caveolin RBBP8/CtIP ENSDARG00000043480 ENSDART00000063832.5 chr24:36315963-36315985,- chr24:36315974 AACTGGGCGTGCAGTGGCGTGGG -0.85 2.57 RBBP8/CtIP ENSDARG00000043480 ENSDART00000063832.5 chr24:36316368-36316390,- chr24:36316379 CTTGCAGTGCGGTGTCGTGCAGG -1.81 2.57 RBBP8/CtIP ENSDARG00000043480 ENSDART00000063832.5 chr24:36316169-36316191,+ chr24:36316180 CGTTTGTGTAAACATTCAGGAGG -2.29 2.57 RBBP8/CtIP ENSDARG00000043480 ENSDART00000063832.5 chr24:36316026-36316048,- chr24:36316037 CTTTGGTTGTTAATGGCTGTAGG 0.1871481962 1.02779950172533 RBBP8/CtIP ENSDARG00000043480 ENSDART00000063832.5 chr24:36315999-36316021,- chr24:36316010 TGAACTTTGCCGCCACCGACTGG 1.713841936 1.02779950172533 RBBP8/CtIP ENSDARG00000043480 ENSDART00000063832.5 chr24:36316092-36316114,- chr24:36316103 CAATATACCTTCAATAATTGGGG -0.4301174415 1.02779950172533 SPRTN ENSDARG00000104709 ENSDART00000158057.1 chr20:3981747-3981769,+ chr20:3981758 CAGACCGAAGAAGATGATGGAGG 0.474 1.511 SPRTN ENSDARG00000104709 ENSDART00000158057.1 chr20:3981823-3981845,- chr20:3981834 TCATCGGGCCATCCAGCAGCCGG -0.508 1.511 SPRTN ENSDARG00000104709 ENSDART00000158057.1 chr20:3981879-3981901,- chr20:3981890 TCACGTCCAAACCACCGGAGGGG 0.625 1.511 SPRTN ENSDARG00000104709 ENSDART00000158057.1 chr20:3981477-3981499,- chr20:3981488 TCACGGAGTGTGAGGTGCGCAGG 0.56 3.013 SPRTN ENSDARG00000104709 ENSDART00000158057.1 chr20:3981597-3981619,- chr20:3981608 ATCAAGTGTTTTCATTAGTGTGG -0.1433 3.013 UCHL-3 ENSDARG00000030177 ENSDART00000104322.5 chr9:22165789-22165811,- chr9:22165800 TTAGCCGGTCTTTATGTCAGTGG 0.9403005336 1.909207574 UCHL-3 ENSDARG00000030177 ENSDART00000104322.5 chr9:22165708-22165730,+ chr9:22165719 CCTCGAGCAATGGTGTGCAGAGG 1.629048297 1.909207574 UCHL-3 ENSDARG00000030177 ENSDART00000104322.5 chr9:22165628-22165650,+ chr9:22165639 CTTCCAGTGGTAACCAGCGCTGG -0.001162998546 1.909207574 USP-11 ENSDARG00000076303 ENSDART00000075624.6 chr8:8605394-8605416,- chr8:8605405 CATTGGGTCTCGAGTCCCGGAGG -0.0530511208 1.241265864 USP-11 ENSDARG00000076303 ENSDART00000075624.6 chr8:8605339-8605361,+ chr8:8605350 TCGGATTTTAGGAGTGATTATGG 0.8043619651 1.241265864 USP-11 ENSDARG00000076303 ENSDART00000075624.6 chr8:8605291-8605313,- chr8:8605302 ACACCGGAATGTTTTGACGAAGG 0.4843152019 1.241265864 USP-11 ENSDARG00000076303 ENSDART00000075624.6 chr8:8605259-8605281,- chr8:8605270 GCGGTTAGAATGACAACTTCCGG 0.25 1.62 USP-11 ENSDARG00000076303 ENSDART00000075624.6 chr8:8605216-8605238,- chr8:8605227 TGGGCGAAACTCCATGGGCGGGG -0.04 1.62 USP-11 ENSDARG00000076303 ENSDART00000075624.6 chr8:8605291-8605313,- chr8:8605302 ACACCGGAATGTTTTGACGAAGG 0.1 1.62 fastkd2 ENSDARG00000074031 ENSDART00000108816.3 chr1:5528379-5528401,- chr1:5528390 GTCACATTTCCACGGTGATGTGG -0.407 1.335 fastkd2 ENSDARG00000074031 ENSDART00000108816.3 chr1:5528370-5528392,+ chr1:5528381 CTCTGAAATCCACATCACCGTGG -0.341 1.335 fastkd2 ENSDARG00000074031 ENSDART00000108816.3 chr1:5528332-5528354,+ chr1:5528343 GAATGTGTATAAATCAGGCTTGG 0.196 1.335 fastkd2 ENSDARG00000074031 ENSDART00000108816.3 chr1:5527734-5527756,- chr1:5527745 GCGCAGCCATGACAGGAGCAGGG 4.457 1.953 fastkd2 ENSDARG00000074031 ENSDART00000108816.3 chr1:5527681-5527703,+ chr1:5527692 AAATCACGTGATACTGAAAGCGG 0.1767 1.953 fastkd2 ENSDARG00000074031 ENSDART00000108816.3 chr1:5527776-5527798,+ chr1:5527787 CATGTGAACGCAACAATCAAAGG 0.53 1.953 fastkd2 ENSDARG00000074031 ENSDART00000145378.2 chr1:5528379-5528401,- chr1:5528390 GTCACATTTCCACGGTGATGTGG -0.407 1.335 fastkd2 ENSDARG00000074031 ENSDART00000145378.2 chr1:5528370-5528392,+ chr1:5528381 CTCTGAAATCCACATCACCGTGG -0.341 1.335 fastkd2 ENSDARG00000074031 ENSDART00000145378.2 chr1:5528332-5528354,+ chr1:5528343 GAATGTGTATAAATCAGGCTTGG 0.196 1.335 fastkd2 ENSDARG00000074031 ENSDART00000145378.2 chr1:5527734-5527756,- chr1:5527745 GCGCAGCCATGACAGGAGCAGGG 4.457 1.953 fastkd2 ENSDARG00000074031 ENSDART00000145378.2 chr1:5527681-5527703,+ chr1:5527692 AAATCACGTGATACTGAAAGCGG 0.1767 1.953 fastkd2 ENSDARG00000074031 ENSDART00000145378.2 chr1:5527776-5527798,+ chr1:5527787 CATGTGAACGCAACAATCAAAGG 0.53 1.953 ATR ENSDARG00000079625 ENSDART00000144801.2 chr2:16780871-16780893,- chr2:16780882 TGCGCGTGGGGAAGAAGCAGAGG 4.22 2.53 ATR ENSDARG00000079625 ENSDART00000144801.2 chr2:16780913-16780935,+ chr2:16780924 CGCACTTTCGCCGTCTACTACGG 1.07 2.53 ATR ENSDARG00000079625 ENSDART00000144801.2 chr2:16780721-16780743,+ chr2:16780732 CTTGCTAATTCTTGCAGTGCAGG -0.18 2.53 ptges ENSDARG00000020136 ENSDART00000026085.6 chr5:32739609-32739631,+ chr5:32739620 GTGCCAAGTATAATGAGGAATGG 0.6166666666666666 2.18 ptges ENSDARG00000020136 ENSDART00000026085.6 chr5:32739664-32739686,- chr5:32739675 TGTAGCATAGTCGTGTTTGGAGG 0.36000000000000004 2.18 ptges ENSDARG00000020136 ENSDART00000026085.6 chr5:32739802-32739824,+ chr5:32739813 CCAAGTGAGACTTCGGAAAAAGG 0.49333333333333335 2.18 slc24a5 ENSDARG00000024771 ENSDART00000033574.5 chr18:5532043-5532065,- chr18:5532054 GCGGCTTACTGAGAGAGAGAGGG 0.8967 2.487 slc24a5 ENSDARG00000024771 ENSDART00000033574.5 chr18:5532093-5532115,- chr18:5532104 ATTCAGCAGAACACAGATGACGG 3.807 2.487 slc24a5 ENSDARG00000024771 ENSDART00000033574.5 chr18:5532121-5532143,+ chr18:5532132 GGACAGACGTGTTTCTCCAGCGG 0.99 2.487 sdc2 ENSDARG00000002731 ENSDART00000141437.2 chr19:47684686-47684708,- chr19:47684697 CTCCGTCAGTAAGACTCCAGAGG -0.4222 1.03 sdc2 ENSDARG00000002731 ENSDART00000141437.2 chr19:47684443-47684465,- chr19:47684454 GCTTTCACGAGCGAAGACTGAGG -0.321 1.03 sdc2 ENSDARG00000002731 ENSDART00000141437.2 chr19:47684526-47684545,- chr19:47684536 AGAGAGCAACACGTATCCGG 0.272 1.03 sdc2 ENSDARG00000002731 ENSDART00000141437.2 chr19:47684684-47684706,+ chr19:47684695 CGCCGCTGGAGTCTTACTGACGG 0.4733 3.013 sdc2 ENSDARG00000002731 ENSDART00000141437.2 chr19:47684645-47684667,+ chr19:47684656 TCTGTGGGCGAGAGTGTGTGTGG 0.81 3.013 sdc2 ENSDARG00000002731 ENSDART00000141437.2 chr19:47684775-47684797,+ chr19:47684786 TCACGTACAGCGCGACCACACGG 0.4033 3.013 sdc4 ENSDARG00000059906 ENSDART00000083830.4 chr6:52677142-52677164,- chr6:52677153 ACGGACAACTTTATTCACTCGGG 0.2586 1.816 sdc4 ENSDARG00000059906 ENSDART00000083830.4 chr6:52677085-52677107,+ chr6:52677096 CCGCCGACAAAACCAACATGAGG 0.2469 1.816 sdc4 ENSDARG00000059906 ENSDART00000083830.4 chr6:52670075-52670097,- chr6:52670086 ACGATGACATGTCAGGCTCTGGG 0.17647 1.816 sdc4 ENSDARG00000059906 ENSDART00000083830.4 chr6:52677299-52677321,- chr6:52677310 AAGGCGATCAATCATTATACAGG 0.9533 1.62 sdc4 ENSDARG00000059906 ENSDART00000083830.4 chr6:52677318-52677340,- chr6:52677329 GAGGGGTTGAATAAAACCAAAGG 1.57 1.62 sdc4 ENSDARG00000059906 ENSDART00000083830.4 chr6:52677224-52677246,- chr6:52677235 CTCAACAAATCGAACACAAAAGG 0.39 1.62 kdr ENSDARG00000017321 ENSDART00000049204.6 chr20:22321364-22321386,+ chr20:22321375 GGTCACTATTTCTGTCGGCAAGG 1.296201107 2.140615296 kdr ENSDARG00000017321 ENSDART00000049204.6 chr20:22321308-22321330,+ chr20:22321319 GCGCACTTCAGTCACGCGTCCGG 1.591026237 2.140615296 kdr ENSDARG00000017321 ENSDART00000049204.6 chr20:22321524-22321546,+ chr20:22321535 CCAGCACTTCTGTCTGGCGATGG 0.80178129 2.140615296 kdr ENSDARG00000017321 ENSDART00000049204.6 chr20:22321327-22321349,- chr20:22321338 TGCTGGACTCGTGCACTGGCCGG 1.983950185 1.38755664239637 kdr ENSDARG00000017321 ENSDART00000049204.6 chr20:22321308-22321330,+ chr20:22321319 GCGCACTTCAGTCACGCGTCCGG 2.735225256 1.38755664239637 kdr ENSDARG00000017321 ENSDART00000049204.6 chr20:22321364-22321386,+ chr20:22321375 GGTCACTATTTCTGTCGGCAAGG 1.255461679 1.38755664239637 flt1 ENSDARG00000019371 ENSDART00000025621.9 chr24:21663629-21663651,- chr24:21663640 GTGTAACTGGAATTCATGCGCGG -0.6907423411 1.201217514 flt1 ENSDARG00000019371 ENSDART00000025621.9 chr24:21663563-21663585,+ chr24:21663574 ACGGGGACAACAGTCAATCTCGG -0.6101946862 1.201217514 flt1 ENSDARG00000019371 ENSDART00000025621.9 chr24:21663374-21663396,- chr24:21663385 TCAGGACGTGTTCTGACTAAAGG -0.4760815921 1.201217514 flt1 ENSDARG00000019371 ENSDART00000025621.9 chr24:21663642-21663664,- chr24:21663653 ATGATGGGAAAGAGTGTAACTGG -0.07969384275 1.71309421551101 flt1 ENSDARG00000019371 ENSDART00000025621.9 chr24:21663596-21663618,- chr24:21663607 TCAAGAGTTCATACACATTGCGG 1.710437824 1.71309421551101 flt1 ENSDARG00000019371 ENSDART00000025621.9 chr24:21663667-21663689,- chr24:21663678 AATGAATAAATCCCAGCCCGCGG -0.3056377107 1.71309421551101 pkd2 ENSDARG00000014098 ENSDART00000020412.7 chr1:49895008-49895030,+ chr1:49895019 CCTCCAGTCTGGAAACAACTGGG 2.434167139 1.551610603 pkd2 ENSDARG00000014098 ENSDART00000020412.7 chr1:49895280-49895302,- chr1:49895291 GATTTCTGTTAAGTCTAACGGGG -0.4761013701 1.551610603 pkd2 ENSDARG00000014098 ENSDART00000020412.7 chr1:49895165-49895187,+ chr1:49895176 TTAGACAGAAAAGCATCCAGCGG 0.3507359964 1.551610603 pkd1a ENSDARG00000030417 ENSDART00000179565.1 chr1:53745824-53745846,- chr1:53745835 GGGTAACTGCTCCTGCACGGCGG 1.909171868 1.074238884 pkd1a ENSDARG00000030417 ENSDART00000179565.1 chr1:53745786-53745808,+ chr1:53745797 GTTGGAGCAATTGACCACGCAGG 1.215888301 1.074238884 pkd1a ENSDARG00000030417 ENSDART00000179565.1 chr1:53745955-53745977,- chr1:53745966 ATTGCGTCTAAGCGAAAACATGG 1.233373515 1.074238884 Rab13 ENSDARG00000034771 ENSDART00000046922.4 chr16:23884789-23884811,+ chr16:23884800 AGGAGCGAAAACTAGCGCAGAGG 1.94 1.5 Rab13 ENSDARG00000034771 ENSDART00000046922.4 chr16:23884812-23884834,+ chr16:23884823 AGCCCGAAATGGCAAATGCGTGG 0.98 1.5 Rab13 ENSDARG00000034771 ENSDART00000046922.4 chr16:23884963-23884985,+ chr16:23884974 CACCGGCGAAAGTTTTCCAGAGG 0.87 1.5 Rab13 ENSDARG00000034771 ENSDART00000046922.4 chr16:23884927-23884949,- chr16:23884938 CTTAGCGGTCTCTGTCCGAAAGG 0.6 1.5 Rab13 ENSDARG00000034771 ENSDART00000046922.4 chr16:23884691-23884713,+ chr16:23884702 GACGTCAGCTTTTGGGAACTCGG 1.3 1.5 Rab13 ENSDARG00000034771 ENSDART00000046922.4 chr16:23884801-23884823,+ chr16:23884812 TAGCGCAGAGGAGCCCGAAATGG 1.48 1.5 Rab13 ENSDARG00000034771 ENSDART00000046922.4 chr16:23884698-23884720,+ chr16:23884709 GCTTTTGGGAACTCGGACAAGGG 1.8 1.5 Rab13 ENSDARG00000034771 ENSDART00000046922.4 chr16:23884779-23884801,- chr16:23884790 AGTTTTCGCTCCTGTATGAGAGG 0.46 1.5 wwp2 ENSDARG00000061345 ENSDART00000087364.5 chr25:34244219-34244241,- chr25:34244230 TCATTTCCGCTTTAATGTCATGG 0.04 2.233 wwp2 ENSDARG00000061345 ENSDART00000087364.5 chr25:34244167-34244189,- chr25:34244178 TGCGGATCCTCTGTAGCATCAGG 0.1367 2.233 wwp2 ENSDARG00000061345 ENSDART00000087364.5 chr25:34244244-34244266,+ chr25:34244255 GTTAGCGCTCCACAGTCTCACGG 1.063 2.233 NRP1a ENSDARG00000102153 ENSDART00000159267.1 chr24:1307942-1307964,+ chr24:1307953 GAGTGAGGAACACAAACATCTGG 0.6667 2.333 NRP1a ENSDARG00000102153 ENSDART00000159267.1 chr24:1307901-1307923,- chr24:1307912 GAACGCCGGGTTACTATACGCGG 0.5267 2.333 NRP1a ENSDARG00000102153 ENSDART00000159267.1 chr24:1307978-1308000,+ chr24:1307989 CATAACAGCGTCTTAAACAAGGG -0.05667 2.333 Hif-1ab ENSDARG00000034293 ENSDART00000018500.8 chr20:20455741-20455763,- chr20:20455752 CGACCAGAAGCTGCACCGACCGG 1.37 1.983 Hif-1ab ENSDARG00000034293 ENSDART00000018500.8 chr20:20455704-20455726,- chr20:20455715 TTTGACCTTCAGCGAACCGGAGG 0.3033 1.983 Hif-1ab ENSDARG00000034293 ENSDART00000018500.8 chr20:20455779-20455801,- chr20:20455790 GAGTAGTCAGAGCGCAGGCACGG 2.743 1.983 Mus81 ENSDARG00000069326 ENSDART00000100782.5 chr7:19598927-19598949,+ chr7:19598938 GACGGAAATGACGAAACGATAGG 0.1967 2.57 Mus81 ENSDARG00000069326 ENSDART00000100782.5 chr7:19598891-19598913,- chr7:19598902 CACGTGTAGCGCCACTCAAAGGG 0.2 2.57 Mus81 ENSDARG00000069326 ENSDART00000100782.5 chr7:19598974-19598996,+ chr7:19598985 ACACAAACAGCGAGCGCGGAAGG 1.137 2.57 Mus81 ENSDARG00000069326 ENSDART00000164266.1 chr7:19598927-19598949,+ chr7:19598938 GACGGAAATGACGAAACGATAGG 0.1967 2.57 Mus81 ENSDARG00000069326 ENSDART00000164266.1 chr7:19598891-19598913,- chr7:19598902 CACGTGTAGCGCCACTCAAAGGG 0.2 2.57 Mus81 ENSDARG00000069326 ENSDART00000164266.1 chr7:19598974-19598996,+ chr7:19598985 ACACAAACAGCGAGCGCGGAAGG 1.137 2.57 ctnnb2 ENSDARG00000023472 ENSDART00000081675.5 chr19:48346969-48346993,- chr19:48346981 CGAGCGCCGCTTTTAAGAAGTGG 0.88 2.333 ctnnb2 ENSDARG00000023472 ENSDART00000081675.5 chr19:48346996-48347018,- chr19:48347007 GCATTCCTCGTATAAGAGCTGGG 0.62 2.333 ctnnb2 ENSDARG00000023472 ENSDART00000081675.5 chr19:48346890-48346912,+ chr19:48346901 CTTGTAAGCAGTGAATCCACCGG 0.66 2.333 btg2 ENSDARG00000020298 ENSDART00000019155.6 chr22:760135-760157,+ chr22:760146 CTGCTCTGATGTGTTGTGAGAGG 0.79 2.74 btg2 ENSDARG00000020298 ENSDART00000019155.6 chr22:760161-760183,- chr22:760172 TCTGCGCGTATATATCGGTGGGG 0.83 2.74 btg2 ENSDARG00000020298 ENSDART00000019155.6 chr22:760089-760111,+ chr22:760100 ATCAATAAACAATCCTCGCGCGG 0.32666666666666666 2.74 tac-1 ENSDARG00000014490 ENSDART00000146947.1 chr19:26065392-26065414,- chr19:26065403 GCGCGTCTCCGTGACCGTTCTGG 1.1666666666666667 2.4833333333333334 tac-1 ENSDARG00000014490 ENSDART00000146947.1 chr19:26065355-26065377,- chr19:26065366 TTTATAGCAAAGCAACAGGGAGG 1.4966666666666668 2.4833333333333334 tac-1 ENSDARG00000014490 ENSDART00000146947.1 chr19:26065432-26065454,+ chr19:26065443 AAGAACTGTGTTATATGTGGAGG 2.486666666666667 2.4833333333333334 ehd1b ENSDARG00000014793 ENSDART00000012717.7 chr10:27106562-27106584,+ chr10:27106573 GCGCAGAAGTTCTCGAGCTGTGG 1.6866666666666668 2.74 ehd1b ENSDARG00000014793 ENSDART00000012717.7 chr10:27106494-27106516,- chr10:27106505 CTATGGACCGGAAACTCGAAGGG 1.5866666666666667 2.74 ehd1b ENSDARG00000014793 ENSDART00000012717.7 chr10:27106594-27106616,+ chr10:27106605 GAGGAGCTGTGCTTTCCGCGCGG 0.9366666666666666 2.74 icn ENSDARG00000009978 ENSDART00000004679.7 chr16:23516139-23516161,- chr16:23516150 CGAGTGAAGACTGACCAAAGAGG 4.083333333333333 1.53 icn ENSDARG00000009978 ENSDART00000004679.7 chr16:23516175-23516197,- chr16:23516186 CACGATGGGCAACAAGTGAAAGG 1.28 1.53 atp5fa1 ENSDARG00000010149 ENSDART00000024199.7 chr21:4916224-4916246,- chr21:4916235 GAGCGCGAGGAGCGGAGTGAAGG 1.31 1.616666666666667 atp5fa1 ENSDARG00000010149 ENSDART00000024199.7 chr21:4916138-4916160,+ chr21:4916149 AATAAAAGTCTCGCGTCGCCTGG 0.31 1.616666666666667 atp5fa1 ENSDARG00000010149 ENSDART00000024199.7 chr21:4916250-4916272,- chr21:4916261 TAGTGTCCTCCTGCAGCGGCCGG 0.9666666666666667 1.616666666666667 ubr5 ENSDARG00000018192 ENSDART00000134444.2 chr16:52761179-52761201,- chr16:52761190 CTTAAACAGAGTTAAGATGGCGG 0.8300000000000001 1.616666666666667 ubr5 ENSDARG00000018192 ENSDART00000134444.2 chr16:52761056-52761078,+ chr16:52761067 CCCGGGTAAATGATTTTGTGTGG 0.19666666666666668 1.616666666666667 ubr5 ENSDARG00000018192 ENSDART00000134444.2 chr16:52761217-52761239,+ chr16:52761228 TGGAGAGGTTGCGCATGTACGGG 0.02666666666666667 1.616666666666667